The identification and characterization of an uptake system for taurine into rat lung slices
Identifieur interne : 003052 ( Main/Exploration ); précédent : 003051; suivant : 003053The identification and characterization of an uptake system for taurine into rat lung slices
Auteurs : Christian P. L. Lewis [Royaume-Uni] ; Gerald M. Cohen [Royaume-Uni] ; Lewis L. Smith [Royaume-Uni]Source :
- Biochemical Pharmacology [ 0006-2952 ] ; 1990.
Descripteurs français
- KwdFr :
- Adénosine triphosphate (pharmacologie), Animaux, Antimycine A (pharmacologie), Chloroquine (pharmacologie), Chlorpromazine (pharmacologie), Cinétique, Colforsine (pharmacologie), Cyanure de potassium (pharmacologie), Dibutyryl AMP cyclique (pharmacologie), Fixation compétitive, Lignées consanguines de rats, Mâle, Poumon (), Poumon (métabolisme), Rats, Sodium (pharmacologie), Taurine (métabolisme), Tétraméthyl-diazènedicarboxamide (pharmacologie), Xanthine(isobutyl-3 methyl-1) (pharmacologie).
- MESH :
- métabolisme : Poumon, Taurine.
- pharmacologie : Adénosine triphosphate, Antimycine A, Chloroquine, Chlorpromazine, Colforsine, Cyanure de potassium, Dibutyryl AMP cyclique, Sodium, Tétraméthyl-diazènedicarboxamide, Xanthine(isobutyl-3 methyl-1).
- Animaux, Cinétique, Fixation compétitive, Lignées consanguines de rats, Mâle, Poumon, Rats.
English descriptors
- KwdEn :
- 1-Methyl-3-isobutylxanthine (pharmacology), Adenosine Triphosphate (pharmacology), Animals, Antimycin A (pharmacology), Binding, Competitive, Bucladesine (pharmacology), Chloroquine (pharmacology), Chlorpromazine (pharmacology), Colforsin (pharmacology), Diamide (pharmacology), Kinetics, Lung (drug effects), Lung (metabolism), Male, Potassium Cyanide (pharmacology), Rats, Rats, Inbred Strains, Sodium (pharmacology), Taurine (metabolism).
- MESH :
- chemical , metabolism : Taurine.
- chemical , pharmacology : 1-Methyl-3-isobutylxanthine, Adenosine Triphosphate, Antimycin A, Bucladesine, Chloroquine, Chlorpromazine, Colforsin, Diamide, Potassium Cyanide, Sodium.
- drug effects : Lung.
- metabolism : Lung.
- Teeft :
- Animals, Binding, Competitive, Biochem, Cell types, Cltaurine, Cltaurine uptake, Cyclic, Cystamine, Diamide, Hypotaurine, Inhibitor, Kidney cortex, Kinetics, Lung slice, Lung slices, Lymphoblastoid cells, Male, Metabolic inhibitors, Polytron vortex homogenizer, Radiochemical techniques, Rats, Rats, Inbred Strains, Saline perfusion, Saturation kinetics, Slice, Structural analogues, Substrate binding, Supernatant fraction, Taurine, Taurine biosynthesis, Taurine transport, Taurine uptake, Transport system, Uptake, Uptake system, Various drugs.
Abstract
Abstract: The objective of this study was to determine whether taurine was accumulated by rat lung slices and if so, to establish the role of this uptake as a source of pulmonary taurine. We have shown that taurine is accumulated into rat lung by an active uptake process that was both ATP and Na+-dependent and obeyed saturation kinetics, exhibiting an apparent Km of 186 μM and Vmax of 970 nmol/ g wet wt/hr. Substrate specificity of the system was high and only compounds possessing anionic and cationic groups separated by two methylene groups were able to competitively inhibit taurine uptake. Subsequent to its uptake, taurine was not significantly metabolized, and since the apparent Km for the uptake process is similar to the known plasma concentration of taurine, it can be inferred that this system will contribute to pulmonary taurine uptake in vivo. Taurine has been suggested to possess antioxidant and antunnammatory properties, and we suggest that this uptake system may contribute to the defence of pulmonary tissue against oxidative stress.
Url:
DOI: 10.1016/0006-2952(90)90047-O
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001B97
- to stream Istex, to step Curation: 001B97
- to stream Istex, to step Checkpoint: 001E23
- to stream Main, to step Merge: 003121
- to stream PubMed, to step Corpus: 000557
- to stream PubMed, to step Curation: 000557
- to stream PubMed, to step Checkpoint: 000531
- to stream Ncbi, to step Merge: 000092
- to stream Ncbi, to step Curation: 000092
- to stream Ncbi, to step Checkpoint: 000092
- to stream Main, to step Merge: 003066
- to stream Main, to step Curation: 003052
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">The identification and characterization of an uptake system for taurine into rat lung slices</title><author><name sortKey="Lewis, Christian P L" sort="Lewis, Christian P L" uniqKey="Lewis C" first="Christian P. L." last="Lewis">Christian P. L. Lewis</name></author><author><name sortKey="Cohen, Gerald M" sort="Cohen, Gerald M" uniqKey="Cohen G" first="Gerald M." last="Cohen">Gerald M. Cohen</name></author><author><name sortKey="Smith, Lewis L" sort="Smith, Lewis L" uniqKey="Smith L" first="Lewis L." last="Smith">Lewis L. Smith</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:E731F7DC12195831FD1C1CBF4983ACF24F669840</idno><date when="1990" year="1990">1990</date><idno type="doi">10.1016/0006-2952(90)90047-O</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-G2JJG8JD-C/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001B97</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001B97</idno><idno type="wicri:Area/Istex/Curation">001B97</idno><idno type="wicri:Area/Istex/Checkpoint">001E23</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001E23</idno><idno type="wicri:doubleKey">0006-2952:1990:Lewis C:the:identification:and</idno><idno type="wicri:Area/Main/Merge">003121</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:1689575</idno><idno type="wicri:Area/PubMed/Corpus">000557</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000557</idno><idno type="wicri:Area/PubMed/Curation">000557</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000557</idno><idno type="wicri:Area/PubMed/Checkpoint">000531</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000531</idno><idno type="wicri:Area/Ncbi/Merge">000092</idno><idno type="wicri:Area/Ncbi/Curation">000092</idno><idno type="wicri:Area/Ncbi/Checkpoint">000092</idno><idno type="wicri:doubleKey">0006-2952:1990:Lewis C:the:identification:and</idno><idno type="wicri:Area/Main/Merge">003066</idno><idno type="wicri:Area/Main/Curation">003052</idno><idno type="wicri:Area/Main/Exploration">003052</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">The identification and characterization of an uptake system for taurine into rat lung slices</title><author><name sortKey="Lewis, Christian P L" sort="Lewis, Christian P L" uniqKey="Lewis C" first="Christian P. L." last="Lewis">Christian P. L. Lewis</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Biochemical Toxicology Section, Central Toxicology Laboratory, Imperial Chemical Industries PLC, Alderley Park, Macclesfield</wicri:regionArea><wicri:noRegion>Macclesfield</wicri:noRegion></affiliation></author><author><name sortKey="Cohen, Gerald M" sort="Cohen, Gerald M" uniqKey="Cohen G" first="Gerald M." last="Cohen">Gerald M. Cohen</name><affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Toxicology Unit, Department of Pharmacology, School of Pharmacy, University of London, 29/39 Brunswick Square, London WC1N 1AX</wicri:regionArea><orgName type="university">Université de Londres</orgName><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Smith, Lewis L" sort="Smith, Lewis L" uniqKey="Smith L" first="Lewis L." last="Smith">Lewis L. Smith</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Biochemical Toxicology Section, Central Toxicology Laboratory, Imperial Chemical Industries PLC, Alderley Park, Macclesfield</wicri:regionArea><wicri:noRegion>Macclesfield</wicri:noRegion></affiliation><affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Biochemical Pharmacology</title><title level="j" type="abbrev">BCP</title><idno type="ISSN">0006-2952</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1990">1990</date><biblScope unit="volume">39</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="431">431</biblScope><biblScope unit="page" to="437">437</biblScope></imprint><idno type="ISSN">0006-2952</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0006-2952</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>1-Methyl-3-isobutylxanthine (pharmacology)</term><term>Adenosine Triphosphate (pharmacology)</term><term>Animals</term><term>Antimycin A (pharmacology)</term><term>Binding, Competitive</term><term>Bucladesine (pharmacology)</term><term>Chloroquine (pharmacology)</term><term>Chlorpromazine (pharmacology)</term><term>Colforsin (pharmacology)</term><term>Diamide (pharmacology)</term><term>Kinetics</term><term>Lung (drug effects)</term><term>Lung (metabolism)</term><term>Male</term><term>Potassium Cyanide (pharmacology)</term><term>Rats</term><term>Rats, Inbred Strains</term><term>Sodium (pharmacology)</term><term>Taurine (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adénosine triphosphate (pharmacologie)</term><term>Animaux</term><term>Antimycine A (pharmacologie)</term><term>Chloroquine (pharmacologie)</term><term>Chlorpromazine (pharmacologie)</term><term>Cinétique</term><term>Colforsine (pharmacologie)</term><term>Cyanure de potassium (pharmacologie)</term><term>Dibutyryl AMP cyclique (pharmacologie)</term><term>Fixation compétitive</term><term>Lignées consanguines de rats</term><term>Mâle</term><term>Poumon ()</term><term>Poumon (métabolisme)</term><term>Rats</term><term>Sodium (pharmacologie)</term><term>Taurine (métabolisme)</term><term>Tétraméthyl-diazènedicarboxamide (pharmacologie)</term><term>Xanthine(isobutyl-3 methyl-1) (pharmacologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Taurine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>1-Methyl-3-isobutylxanthine</term><term>Adenosine Triphosphate</term><term>Antimycin A</term><term>Bucladesine</term><term>Chloroquine</term><term>Chlorpromazine</term><term>Colforsin</term><term>Diamide</term><term>Potassium Cyanide</term><term>Sodium</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Poumon</term><term>Taurine</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Adénosine triphosphate</term><term>Antimycine A</term><term>Chloroquine</term><term>Chlorpromazine</term><term>Colforsine</term><term>Cyanure de potassium</term><term>Dibutyryl AMP cyclique</term><term>Sodium</term><term>Tétraméthyl-diazènedicarboxamide</term><term>Xanthine(isobutyl-3 methyl-1)</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Animals</term><term>Binding, Competitive</term><term>Biochem</term><term>Cell types</term><term>Cltaurine</term><term>Cltaurine uptake</term><term>Cyclic</term><term>Cystamine</term><term>Diamide</term><term>Hypotaurine</term><term>Inhibitor</term><term>Kidney cortex</term><term>Kinetics</term><term>Lung slice</term><term>Lung slices</term><term>Lymphoblastoid cells</term><term>Male</term><term>Metabolic inhibitors</term><term>Polytron vortex homogenizer</term><term>Radiochemical techniques</term><term>Rats</term><term>Rats, Inbred Strains</term><term>Saline perfusion</term><term>Saturation kinetics</term><term>Slice</term><term>Structural analogues</term><term>Substrate binding</term><term>Supernatant fraction</term><term>Taurine</term><term>Taurine biosynthesis</term><term>Taurine transport</term><term>Taurine uptake</term><term>Transport system</term><term>Uptake</term><term>Uptake system</term><term>Various drugs</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cinétique</term><term>Fixation compétitive</term><term>Lignées consanguines de rats</term><term>Mâle</term><term>Poumon</term><term>Rats</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The objective of this study was to determine whether taurine was accumulated by rat lung slices and if so, to establish the role of this uptake as a source of pulmonary taurine. We have shown that taurine is accumulated into rat lung by an active uptake process that was both ATP and Na+-dependent and obeyed saturation kinetics, exhibiting an apparent Km of 186 μM and Vmax of 970 nmol/ g wet wt/hr. Substrate specificity of the system was high and only compounds possessing anionic and cationic groups separated by two methylene groups were able to competitively inhibit taurine uptake. Subsequent to its uptake, taurine was not significantly metabolized, and since the apparent Km for the uptake process is similar to the known plasma concentration of taurine, it can be inferred that this system will contribute to pulmonary taurine uptake in vivo. Taurine has been suggested to possess antioxidant and antunnammatory properties, and we suggest that this uptake system may contribute to the defence of pulmonary tissue against oxidative stress.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Grand Londres</li></region><settlement><li>Londres</li></settlement><orgName><li>Université de Londres</li></orgName></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Lewis, Christian P L" sort="Lewis, Christian P L" uniqKey="Lewis C" first="Christian P. L." last="Lewis">Christian P. L. Lewis</name></noRegion><name sortKey="Cohen, Gerald M" sort="Cohen, Gerald M" uniqKey="Cohen G" first="Gerald M." last="Cohen">Gerald M. Cohen</name><name sortKey="Smith, Lewis L" sort="Smith, Lewis L" uniqKey="Smith L" first="Lewis L." last="Smith">Lewis L. Smith</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003052 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003052 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:E731F7DC12195831FD1C1CBF4983ACF24F669840
|texte= The identification and characterization of an uptake system for taurine into rat lung slices
}}
| This area was generated with Dilib version V0.6.33. |  |